Rapid detection of Escherichia coli O157:H7 with multiplex real-time PCR assays.
نویسندگان
چکیده
A SYBR Green LightCycler PCR assay using a single primer pair allowed simultaneous detection of stx1 and/or stx2 of Escherichia coli O157:H7. A distinct sequence of the Shiga-like toxin genes was amplified to yield products of 227 and/or 224 bp, respectively. The two products were distinguished by melting point curve analysis.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 68 6 شماره
صفحات -
تاریخ انتشار 2002